A NEW ROBUST ANALYTICAL METHOD DEVELOPMENT, VALIDATION, AND STRESS DEGRADATION STUDIES FOR ESTIMATING RITONAVIR BY UV-SPECTROSCOPY AND HPLC METHODS
نویسندگان
چکیده
Objective: To specify ritonavir and its commercial dosage form, the current study set out to design validate concise, precise, efficient high-performance liquid chromatography spectrophotometric methods. The developed spectroscopy chromatographic methods are reliable, accurate, specific for estimating ritonavir. Methods: method is superior previously described due shorter retention duration, use of an affordable easily accessible mobile phase, UV detection, improved peak resolution. maximum absorbance was determined by analysing multiple concentration ranges at 10-60 µg/ml using UV-spectrophotometric method. separation performed with a phase composed acetonitrile 0.1% formic acid (1:1 v/v) pumped 1.0 ml/min flow rate on phenyl (150 x 4.6 mm, 3.5 µm) column. Results: Obeyed Beer-Lambert law over 25-150 range HPLC methods, respectively. 273 nm selected as throughout UV-spectroscopic study. detection quantification limits 0.89 2.93 µg/ml, whereas 0.78 2.57 In accuracy precision validation studies, amount recovery percentage RSD excellent acceptance per International Conference Harmonization (ICH) guidelines. Conclusion: suggested has been approved following standards established ICH. can be employed analyse API pharmaceutical forms provide better specificity, separation, specified analyte degradation substances.
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International Journal of Pharmaceutical Sciences Review and Research Available online at www.globalresearchonline.net © Copyright protected. Unauthorised republication, reproduction, distribution, dissemination and copying of this document in whole or in part is strictly prohibited. © Copyright protected. 16 Saroj Jain, Anupama Diwan, Satish Sardana Department of Pharmaceutics, Hindu College of...
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ژورنال
عنوان ژورنال: International Journal of Applied Pharmaceutics
سال: 2023
ISSN: ['0975-7058']
DOI: https://doi.org/10.22159/ijap.2023v15i4.47924